Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here. we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry. suitable to specifically map the content of autophagosomes and potentially other short-lived endomembrane transport vesicles without the... https://www.markbroyard.com/hot-save-turbo-air-m3f24-1-n-29-wide-m3-series-reach-in-freezer-featuring-one-solid-door-energy-star-on-sale-top-super/